Note: Cryopreservation
of common carp sperm
Á. Horváth, E. Miskolczi, B. Urbányi-2003
Abstract:
Experiments were
carried out to investigate the effect of five extenders (sucrose, glucose,
fructose, KCl and a saline carp sperm extender) and two cryoprotectants
(dimethyl-sulfoxide (DMSO) and methanol) on the cryopreservation of common
carp sperm. Freezing of sperm using glucose extender and methanol as
cryoprotectant resulted in the highest post-thaw motility, fertilization as
well as hatching rates (63 ± 9%, 74 ± 15% and 67 ± 17%
vs. 87 ± 5%, 84 ± 14% and 69 ± 14% using
fresh sperm, respectively). In general, sugar-based extenders combined with
methanol as cryoprotectant yielded higher motility, fertilization and
hatching rates than ionic extenders in combination with DMSO. The jelly-like
agglutination observed after thawing in samples frozen with sugar-based
extenders did not reduce fertilization and hatching rates. Frozen–thawed
sperm samples were able to successfully fertilize 10 g (8000) eggs.
(Department of Fish Culture, Szent István
University, Páter K.u. 1, 2103, Gödöllő, Hungary, e-mail: ahorvath@kgi.gau.hu)