The immunohistochemical study of nodavirus changes in larval,
juvenile and adult sea bass tissue
I. Mladineo-2003
Journal of
Applied Ichthyology, 19(6):
366
Summary:
Owing to its quickness and sensitivity,
and the minimal financial effort required, immunohistochemistry is a leading
technique for nodavirus diagnosis. The aim of the study was to give detailed
results of immunohistochemical changes in the various tissues of larvae,
juvenile and adult sea bass (Dicentrarchus labrax L.). Histological
sections were treated with polyclonal anti-noda rabbit IgG, while
diaminobenzidine-peroxidase was used for the enzyme-substrate complex. The
development from first changes to vacuolating necrosis was recorded in the
brain, spinal cord and retina. For the first time, immunolabelling was noted
in the liver endothelium. The other sampled tissue was immunonegative.
(Institute of Oceanography and Fisheries,
P.O. Box 500, 21000 Split, Croatia, E-mail: mladineo@izor.hr)