Gynogenesis Assessment Using
Microsatellite Genetic Markers in Turbot (Scophthalmus maximus)
J. Castro, C. Bouza, L. Sánchez, R.M. Cal,
F. Piferrer, P. Martínez
Marine Biotechnology, 5(6): 584-592
Abstract:
Gynogenesis was assessed by different methods in 2
families of gynogenetic offspring in turbot (Scophthalmus maximus).
Karyotype analysis in embryos and larvae demonstrated high accuracy in
estimation of ploidy level, but performance was uneven given the low quality
and number of plates obtained. The use of silver staining to estimate the
number of nucleoli per nucleus resulted in a straightforward and easy method
to evaluate the ploidy of the samples studied. However, the existence of a
nucleolus organizer region polymorphism in turbot determined a small error
in ploidy estimation, important when checking ploidy in specific
individuals. The use of a set of 11 highly variable microsatellite loci
proved to be a powerful method to confirm the exclusive maternal inheritance
to gynogenetic offspring in turbot, with probabilities of detection of
putative paternal genetic contribution above 99.99%.
(Departamento de Genética,
Universidad de Santiago de Compostela, 27002 Lugo, Spain, e-mail of P.
Martinez: paumarpo@lugo.usc.es)