Induced
spermiation in 3-year-old sterlet, Acipenser ruthenus L.
A. Rzemieniecki, J. Domagałła,
J. Glogowski, A. Ciereszko, R. Trzebiatowski, J. Kouřil, J. Hamáčkova,
I. Babiak-2004
Aquaculture
Research, 35(2):
144-151
Abstract:
Spermiation in 3-year-old sterlet, Acipenser
ruthenus (L.), males maintained under warm water conditions was induced
by intramuscular injection of either (i) (D-Ala6)-GnRH-ProNHEt
(Kobarelin); (ii) mammalian GnRH analogue+metoclopramide (Ovopel); or (iii)
human chorionic gonadotropin (Biogonadyl). The volume of milt, sperm
concentration and motility were measured. A higher percentage of spermiating
males was obtained after Kobarelin or Ovopel treatment (81.8% and 77.7%
respectively) in comparison with fish treated with Biogonadyl (40.0%).
However, only stimulation with Ovopel guaranteed motile spermatozoa in all
spermiating males. Moreover, treatment with Ovopel resulted in the highest
average milt volume, sperm concentration and motility. The average total
number of motile spermatozoa (milt volume x sperm
concentration x 109, 5.31 x 109 after stimulation with Kobarelin, Ovopel or Biogonadyl respectively. Our
data indicate that Ovopel is a good stimulator of spermation in sturgeons.
Moreover, the time of sexual maturation could be significantly reduced in
sturgeons maintained in cages under warm water conditions.
(Department of General Zoology, Szczecin
University, Felczaka 3a, 71-412 Szczecin, Poland. E-mail: Adam.Rzemieniecki@univ.szczecin.pl)