An RFLP database for
authentication of commercial cyst samples of the brine shrimp Artemia
spp. (International Study on Artemia LXX)
P.
Bossier, W. Xiaomei, F. Catania, S. Dooms, G. Van Stappen, E. Naessens, P.
Sorgeloos-2004
Aquaculture, 231(1-4): 93-112
Abstract:
Artemia cysts, originating from a variety of locations, are
currently commercially exploited. The overall quality of the cysts depends
on a lot of features including their intrinsic nutritional quality,
harvesting and processing conditions, diapause characteristics and size, all
influencing their commercial value. In order to mediate authentication of
commercial Artemia cyst samples, a database containing eight RFLP
patterns of a 1500 bp mitochondrial rDNA fragment was constructed. The
database contains 53 samples, covering most of the geographical distribution
of Artemia. On the basis of a band sharing index these 53 samples
could be clustered into five groups. These groups coincide to a great extent
with the currently accepted species or species complexes. Within each
cluster diversity between samples is still considerable, reflecting the
genetic diversity within each species. The developed method allows to assign
samples to these clusters, facilitating their authentication at the species
level.