Advances in octopus culture: I. Reproduction


European Network for the Dissemination of Aquaculture RTD Information (Q5CA-2000-30105) and previously FAIR-3837, Aquaflow ref. : TL2003-191

The common octopus, Octopus vulgaris, is found world wide, from near shore shallows to as deep as 150m. It is a species with a relatively short life cycle (approximately 2 years). Following hatching, the paralarvae spend the first weeks of their life in the plankton. Those that survive settle out and begin a benthic (bottom dwelling) life.

O. vulgaris is commercially important and accounts for a large percentage of octopus fisheries. The common octopus also meets several of the criteria to be considered as a candidate for industrial rearing: easy adaptation to captivity, high growth rates (2-4% of body weight per day at 20°C), high fecundity, and high economic value. Embryonic development and the description of the early developmental stages have been performed both in the wild and under laboratory conditions. Several trials have also been conducted for the breeding and ongrowing of octopus at industrial level. The objectives of the current project were to obtain paralarvae from broodstock kept in captivity, to complete the early developmental stages, and to study the growth pattern of octopus during the ongrowing phase, towards commercialization of this species.

Individuals of the common octopus were caught at the Saronicos Bay (Attiki, Greece), sexed, transferred to the installations of the Zoology-Marine Biology Laboratory (Biology Department, University of Athens), and placed into 200 l aquariums under a recirculating water system. Reproduction experiments were conducted at a salinity of 37‰ and temperature 20°C.  Once the spawns were obtained, the number of egg clusters, average length, and the number of eggs per cluster was counted. Embryonic development was followed in two spawns.

Results indicated that a single male can fertilise many females and that it is possible that octopus can mate more than once in their life span.  Egg-bearing females tend to avoid males. Each female deposits clusters of eggs, at a daily ratio of 10-20 clusters, for a period of 4 to 6 weeks. However, egg deposit only takes place water temperatures above 10°C.  Spawning was monitored in two females. The first female (197g), produced 9,000 eggs, hatching was partial and lasted for 120h. The second female (238g), produced 6,500 eggs, hatching lasted 24 hours and all eggs were hatched. During this period, i.e. between spawning and egg hatching, the females stay close to the cluster of eggs, protecting them. The females stopped feeding and showed a weight loss of 14-28% of their body weight. The females died 20 days after hatching. Newly deposit eggs had an average length of 2.71mm and an average width of 1.25mm. Following hatching planktonic larvae were placed in mesocosms containing phytoplanktonic and zooplanktonic organisms (Dunalliela tertiolecta, Chlorella mininutissima, Brachionus plicatilis, Artemia salina and wild zooplanktonic strains). Under these conditions the paralarvae increased their size, but showed low survival rates. At the end of the experimental larvae rearing mortality was 100%. According to the literature larvae rearing with several live diets usually results in 100% mortality and in only one case 5% survival after two months of life.

These preliminary results are similar with other studies on the artificial propagation of octopus. Reproduction in captivity is feasible but research should be focused on the quality and the nutritional profile of the prey for the early developmental stages.

The project is co-financed by the Ministry of Development, General Secretariat for R&D (G.S.R.D.); co-financing project – 50% G.S.R.D. and 50% Aquaculture Centre of Acheloos S.A. (ICHTYKA).  The Biology Department of the University of Athens is implementing the project.

For more information, contact:

G. Verriopoulos
University of Athens,Department of Biology
Greece
Tel.: (030-1)7274 608 - Fax: (030-1)7274 604
E-mail gverriop@biol.uoa.gr


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