Successful Hybridization of Acipenser
Species Using Cryopreserved Sperm
B. Urbányi,
Á.
Horváth,
B. Kovács-2004
Aquaculture International: 12(1):
47-56
Abstract:
Successful hybridization of sterlet (Acipenser
ruthenus) × Siberian sturgeon (Acipenser
baeri), sterlet × Russian sturgeon (Acipenser
gueldenstaedti) and sterlet × European
sturgeon (Acipenser sturio) was carried out for the first time
using cryopreserved semen of sturgeon males and sterlet ×
sterlet crosses as controls. Sperm of all three species was diluted with a
cryodiluent composed of 23.4 mM sucrose, 0.25 mM KCl, 30 mM Tris (pH 8.0)
and 10% methanol. The samples were frozen in plastic straws in the vapor of
liquid nitrogen at the height of 3 cm above the level of nitrogen for 3 min.
Following thawing approximately 3000 sterlet eggs were fertilized with six
straws of frozen-thawed sperm. The hatching rate with sterlet sperm was
30.6% while the hatching rate of A. ruthenus ×
A. baeri, A. ruthenus × A.
gueldenstaedti and A. ruthenus ×
A. sturio hybrids was 50, 17.4 and 34%, respectively. Morphometric
markers as well as random amplified polymorphic DNA (RAPD) assay was used to
verify interspecific hybridization.
(Department of Fish Culture, Faculty
of Agriculture and Environmental Sciences, Szent Istvan University, Páter
K. u. 1., Gödöllö,
H-2103, Hungary, Tel: +36-28-522-000/1657; fax: +36-28-410-804, e-mail: urbanyib@nt.ktg.gau.hu)