Exhaustion of lipid reserves
in the hepatopancreas of Fenneropenaeus indicus broodstock in
relation to successive spawnings
C.G. Vazquez Boucard, J. Patrois, H.J. Ceccaldi-2004
Aquaculture, 236(1-4): 523-537
Abstract:
After eyestalk ablation, penaeid shrimp females spawn
several times during a reproductive cycle. This forced reproduction affects
larval quality in production laboratories. The biochemical composition of
ovaries and hepatopancreas (total proteins, total lipids, vitellin, lipid
and fatty acids classes) of Fenneropenaeus indicus spawners was
studied according to spawning rank. Depletion of the total protein and lipid
hepatopancreas reserves was observed before the fourth spawning (total
proteins down from 99.89 to 26.13 mg/hepatopancreas, total lipids down from
133.38 to 26.92 mg/hepatopancreas). Structural lipids (phospholipids),
energy lipids (triacylglycerols) and some minor classes of lipids (sterol,
free fatty acids, diacylglycerols and hydrocarbons) also decreased
significantly. The successive spawning also affected some biochemical
compounds of the ovary (total proteins down from 291.62 to 256.46 mg/ovary,
total lipids down from 101.07 to 73.63 mg/ovary, vitellin down from 373.48
to 302.77 mg/ovary, triacylglycerols down from 35.80% to 25.78% of total
lipids). This decrease is related to the size of ovaries since the
gonadosomatic index (GSI) fell by about 20% between the first and the fourth
spawning. This study demonstrated depletion of nutritional reserves in
hepatopancreas of spawning shrimp.
(Department of Marine Aquaculture, Centro de
Investigaciones Biologicas del Noroeste (CIBNOR), La Paz, BCS, Mexico,
e-mail of C.G. Vazquez Boucard: cboucard@cibnor.mx,
e-mail of H.J. Ceccaldi: hubert.ceccaldi@CERAM.u-3mrs.fr)