Combinations of
lactic acid bacteria and yeast suitable for preparation of marine silage
M. Uchida, H. Amakasu, Y. Satoh, M.
Murata-2004
Fisheries
Science, 70(3):
507-517
Abstract:
Marine silage (MS) is a fish hatchery diet
prepared by decomposing seaweed into cellular units and performing lactic
acid fermentation. MS was first developed by using a wild-type consortium
composed of a lactic acid bacterium (LAB) Lactobacillus brevis and
yeasts Debaryomyces hansenii var. hansenii and Candida
sp. as a fermentation starter. To further examine a suitable combination of
starter microorganisms, MS was prepared with different compositions of LAB
and yeasts from a substrate of Undaria pinnatifida. Production
efficiency of algal single cell units was not different among the trials.
Inoculation of LAB significantly retarded the growth of contaminant
microorganisms while a single inoculation of yeasts could not retard the
growth of halophilic contaminant microorganisms. Lactobacillus brevis
showed the highest predominating ability in MS among the tested, while L. acidophilus
(casei-type) IAM 10074 and L. plantarum IAM12477T
also showed predominating ability with relatively inferior scores when
analyzed from the results of the identification with 16S rRNA-targeted
species-specific primers. The present study demonstrated that a single use
of LAB, including food industry-familiar species, is suitable for
preparation of MS, while the superiority of L. brevis B5201 was
suggested in predominating ability in marine silage.
(National Research Institute of Fisheries and
Environment of Inland Sea, Fisheries Research Agency, Ohno, Hiroshima
739-0452, Japan, Tel: 81-829-55-3430. Fax: 81-829-54-1216. Email: uchida@affrc.go.jp)