Induced Ovulation in Obscure
Puffer Takifugu obscurus by Injections of LHRH-a
Zhou Yang, Ya-Fen Chen-2004
Aquaculture International, 12
(2): 215-223
Abstract:
Obscure puffer Takifugu obscurus (Abedate) is
one of the several species of Takifugu genus that migrate to the
Yangtze River, China during the spawning season of February to May. It is a
popular fish among locals and fetches a premium price owing to its
high-quality meat. It is also a potential species for intensive culture as
its natural populations are currently declining. There have been no
published studies on the effects of GnRH-a injection on the obscure puffer.
In this paper, we report the results of several obscure puffer ovulation
trials conducted in 1995 and 1996. We demonstrated that ovulation could
successfully be induced in obscure puffer collected from open systems during
spawning period using a dosage of LHRH-a as low as 40 µg
kg-1 in two injections. We found no significant differences among
80, 60, 40 µg LHRH-a kg-1
treatments in ovulation success, egg production, fertilization rate and
hatch rate. Accordingly, we recommend the use of 40 µg
LHRH-a kg-1 as the optimal dosage from an economical point of
view.
(School of
Biological Science, Nanjing Normal University, 122 Ninghai Road, 210097
Nanjing, P.R. China, e-mail: yangzhouff@vip.sina.com)