Daily change of
genetic variability in hatchery offspring of red sea bream during spawning
season
E. Nugrohoa, N. Taniguchi-2004
Fisheries
Science, 70(4):
638-644
Abstract:
In order to assess a daily change of
genetic variability during spawning season, hatched larvae of red sea bream
sampled on different dates were assayed by polymorphic markers such as
microsatellite DNA (msDNA) and mitochondria DNA (mtDNA) control region.
Based on the microsatellite loci, the average number of alleles per locus
ranged between 13.7 and 18.3. The expected heterozygosities ranged between
0.843 and 0.919. A total of 23 mtDNA haplotypes were detected via digestion
of mtDNA D-loop sequences with five endonucleases: Taq I, Alu I,
Mbo I, Rsa I and Hinf I. Significant
fluctuation of genetic variability during spawning season was detected by
both types of DNA markers. It was suggested that the genetic variability was
maintained by pooling the seed fish collected on different spawning dates in
a hatchery.