Cryopreservation of sperm of
farmed European eel Anguilla anguilla
T; Muller, B. Urbanyi, B. Varadi, T. Binder, P. Horn,
M. Bercsenyi, A. Horvath-2004
Journal of the World Aquaculture Society, 35(2):
225-231
Abstract:
Sexual maturation and sperm release were induced in farmed European eels
Anguilla anguilla kept exclusively in fresh water by using two dosages of
human chorion gonadotropin (100 International Unit (IU)-Group one and 250
IU/individual per week-Group two). Sperm release took over 13 wk in both
groups. The quality of sperm was investigated on the eighth, ninth, and
tenth wk. The average cell densities were 0.94 +/- 0.4 x 10(10) (Group one)
and 0.93 +/- 0.6 x 10(10) (Group two) spermatozoa/mL. The estimated motility
of eel sperm was 33, 55, and 49% on the eighth, ninth, and tenth wk of
treatment, respectively. The estimated average motility of samples selected
for cryopreservation was 73 +/- 10%, while the post-thaw motility of
cryopreserved samples was 36 +/- 11%. The extender originally developed for
common carp sperm crypreservation together with methanol as cryoprotectant
was found suitable for the cryopreservation of European eel sperm.
(Univ. Veszprem, Georgikon Fac. Agr., H-8360,
Keszthely, Hungary)