Enhancement of rotifer (Brachionus
plicatilis) growth by using terrestrial lactic acid bacteria
M. Planas, J.A. Vázquez, J. Marqués, R. Pérez-Lomba,
M.P. González, M. Murado-2004
Aquaculture, 240(1-4): 313-329
Abstract:
One of the pathways for the entry of bacteria, both
pathogenic and probiotic, into the larvae of fish hatcheries is via live
prey. As a preventive measure against infections, live prey may be cultured,
supplementing the food with probiotics. Some lactic acid bacteria (LAB) have
been successfully used in the larviculture. In this study, the nutritional
effect of seven terrestrial LAB has been studied regarding the growth of the
rotifer Brachionus plicatilis. The cultures were carried out without
partial renewal of the culture medium, feeding the rotifers on baker's yeast
and adding some of the species of bacteria. In all cases, the addition of
the bacteria increased both the specific maximum growth rate and the maximum
density obtainable in the cultures. However, the best results were obtained
with the addition of Lactococcus casei ssp. casei, Pediococcus
acidilactici, or Lactobacillus lactis spp lactis. The
rates of growth obtained with the individual or joint addition of these
three bacteria were 8–13 times greater than those obtained with the
control cultures after 4–5 days' culture. In this study, a series of
kinetic models has been applied (logistic modified—Gompertz,
logistic–logistic, and generalised logistic) which describes the
experimental data, obtaining a set of parameters of biological significance
which facilitate the optimisation of the use of these bacterial strains in
the mass production of rotifers.