Daily change of genetic variability in hatchery offspring of red sea
bream during spawning season
E.
Nugrohoa, N. Taniguchi-2004
Fisheries
Science, 70(4):
638-644
Abstract:
In
order to assess a daily change of genetic variability during spawning
season, hatched larvae of red sea bream sampled on different dates were
assayed by polymorphic markers such as microsatellite DNA (msDNA) and
mitochondria DNA (mtDNA) control region. Based on the microsatellite loci,
the average number of alleles per locus ranged between 13.7 and 18.3. The
expected heterozygosities ranged between 0.843 and 0.919. A total of 23
mtDNA haplotypes were detected via digestion of mtDNA D-loop sequences with
five endonucleases: Taq I, Alu I, Mbo I,
Rsa I and Hinf I. Significant fluctuation of genetic
variability during spawning season was detected by both types of DNA
markers. It was suggested that the genetic variability was maintained by
pooling the seed fish collected on different spawning dates in a hatchery.
(Laboratory
of Population Genetic Informatics, Graduate School for Agricultural Science,
Tohoku University, Sendai, Miyagi 981-8555, Japan, e-mal of N. Taniguchi: nobuhiko@bios.tohoku.ac.jp)