Applying
microsatellites to the management of farmed turbot stocks (Scophthalmus
maximus L.) in hatcheries
Y.J.
Borrell, J. Álvarez, E. Vázquez, C. Fernández Pato, C. Martínez Tapia,
J.A. Sánchez, G. Blanco-2004
Aquaculture, 241(1-4): 133-150
Abstract:
Eight
microsatellite loci reported previously to exhibit high levels of
variability were assessed for their utility for determining pedigrees in
five turbot (Scophthalmus maximus L.) broodstocks from two hatcheries
in Spain and for estimating genetic relatedness between pairs of individuals
with unknown origin through relatedness coefficients. Discrepancies were
found among expected levels of correct parentage assignment obtained by
simulation procedures (using Cervus or Probmax) and those obtained working
with real descendants. Besides the genetic variability levels found in
broodstocks, other factors such as mutation, occurrence of null alleles and
genetic similarities among parents of the same sex significantly affected
parentage assignment test results. On the other hand, selection of breeding
pairs for avoiding inbreeding in progenies would be possible with more than
80% accuracy using relatedness coefficients below zero as the cut-off
between the selected individuals. In summary, microsatellite markers are
useful tools for parentage studies in hatcheries but their efficacy is not
based only on the genetic variability found.
(Laboratorio
de Genética Acuícola, Departamento de Biología Funcional, Universidad de
Oviedo, 33071 Oviedo, Spain, e-mail of G. Blanco: gloriablanco@uniovi.es)