1.1 : Preparing the Biofilter

1.1.1 Choice of the Substrate
1.1.2 Optimizing Water - Parameter 1 : Temperature
1.1.3 Optimizing Water - Parameter 2 : Salinity
1.1.4 Water Volume
1.1.5 Oxygen
1.1.6 Summary

1.2 : Disinfection of the Biofilter

1.2.1 Choice of the Disinfectant
1.2.2 Detection and Quantification of Sodium Hypochlorite
1.2.2.1 Detection of Hypochlorite in Water
1.2.2.2 Titration of Hypochlorite
1.2.2.2.1 Theoretical Activity of Chlorine
1.2.2.2.2 Determination of the Neutralization Product
1.2.2.2.3 Determination of the Activity
1.2.3 Disinfection of the Biofilter with Sodium Hypochlorite (exercise)
1.2.4 Neutralisation of Sodium Hypochlorite
1.2.4.1 Procedure (exercise)
1.2.4.2 Amount of Sodium Thiosulphate

1.3 : Activation of the Biofilter

1.3.1 Introduction
1.3.2 The Nitrification Proces
1.3.2.A The Nitrification Process
1.3.2.B The Nitrification Process
1.3.3.1 Choice of the Inoculum
1.3.3.2 Conditioning of the biofilter
1.3.3.2.A Conditioning of the biofilter
1.3.3.2.B Conditioning of the biofilter

Part 2 : The Culture of the Rotifer Brachionus Plicatilis